Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation

Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1

Retinal S-antigen Th1 cell epitope mapping in patients with Behcet's disease

Background - Retinal S-antigen (S-Ag) is a most characterized autoantigen of autoimmune uveitis. The recognized immunodominant epitope of human S-Ag in patients with uveitis has not been identified. In this study, we selected certain patients with active uveitis to map the Th1 cell epitope spectrum of human S-Ag in Behcet's disease(BD). Methods - Blood samples were taken from eight active BD patients who showed an immune response to 40 mixed overlapping peptides spanning the entire sequence of human S-Ag. Peripheral blood mononuclear cells were isolated and stimulated with single S-Ag peptide at 5 mu g/ml or 20 mu g/ml. Single-cell immune responses were measured by IFN-gamma ELIspot assay. Results - BD patients heterogeneously responded to the S-Ag peptides at two concentrations. In general, the responses to 5 mu g/ml peptides were slightly stronger than those to 20 mu g/ml peptides, while the maximum SFC frequency to single peptide at the two concentrations was similar. Several peptides including P31, P35 and P40 induced a prominent response, with the frequency of S-Ag specific cells being about 0.007%. Significant reactivity pattern shift was noted in patients with different disease courses. Conclusions - Certain active BD patients have S-Ag specific Th1 cells with a low frequency. The S-Ag epitope specificity between patients is highly heterogeneous, and varies with the uveitis cours

The impacts of magnetic fields on the thermocapillary convection in two layers fluid system

Paper presented at the 5th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, South Africa, 1-4 July, 2007.Under a horizontal temperature gradient along the liquidliquid interface, the developing processes of thermocapillary convection in two layers immiscible fluids system absent gravity were simulated numerically, where the upper layer fluid was encapsulant B2O3, the underlayer fluid was melting InP in this paper. The effects of different direction magnetic field on the developing behaviors of thermocapillary convection were investigated. The results showed that the flow pattern was changed obviously and the thermocapillary convection was damped in some extent and the temperature distributions became more uniform if magnetic fields in X, Y or Z direction were applied. Z direction magnetic field had a stronger effect on the thermocapillary convection and it was enough to suppress convection significantly at Bz between 0.15T and 0.2T. The simulation became numerically unstable when Bz was over 0.2T.cs201

Domain movement in rabbit muscle adenylate kinase might involve proline isomerization

AbstractThe fluorescence probe, 8-anilino-1-naphthalenesulfonic acid (ANS), was used to monitor the induced-fit conformational movement in rabbit muscle adenylate kinase. In 50 mM Tris-HCl buffer (pH 8.1), the time course of ANS binding to rabbit muscle adenylate kinase is a biphasic process. The fast phase completes within the dead-time of the stopped-flow equipment used (about 15 ms), while the slow phase ends in about 10 minutes. In the presence of 2.0 μM peptidyl prolyl cis/trans-isomerase, the rate constant of the slow phase reaction is accelerated about 2.4-fold, suggesting that the domain movement during ANS binding to rabbit muscle adenylate kinase may involve proline isomerization. The activation energy of the slow phase was determined to be 74.6 kJ/mol, which is comparable to the activation energy of proline cis/trans-isomerization (about 80 kJ/mol)

EGAM Induced by Energetic-electrons and Nonlinear Interactions among EGAM, BAEs and Tearing Modes in a Toroidal Plasma

In this letter, it is reported that the first experimental results are associated with the GAM induced by energetic electrons (eEGAM) in HL-2A Ohmic plasma. The energetic-electrons are generated by parallel electric fields during magnetic reconnection associated with tearing mode (TM). The eEGAM localizes in the core plasma, i.e. in the vicinity of q=2 surface, and is very different from one excited by the drift-wave turbulence in the edge plasma. The analysis indicated that the eEGAM is provided with the magnetic components, whose intensities depend on the poloidal angles, and its mode numbers are jm/nj=2/0. Further, there exist intense nonlinear interactions among eEGAM, BAEs and strong tearing modes (TMs). These new findings shed light on the underlying physics mechanism for the excitation of the low frequency (LF) Alfv\'enic and acoustic uctuations.Comment: 5 pages,4 figure

Building Oblivious Transfer on Channel Delays

In the information-theoretic setting, where adversaries have unlimited computational power, the fundamental cryptographic primitive Oblivious Transfer (OT) cannot be securely achieved if the parties are communicating over a clear channel. To preserve secrecy and security, the players have to rely on noise in the communication. Noisy channels are therefore a useful tool to model noise behavior and build protocols implementing OT. This paper explores a source of errors that is inherently present in practically any transmission medium, but has been scarcely studied in this context: delays in the communication. In order to have a model for the delays that is both general and comparable to the channels usually used for OT – such as the Binary Symmetric Channel (BSC) – we introduce a new noisy channel, the Binary Discrete-time Delaying Channel (BDDC). We show that such a channel realistically reproduces real-life communication scenarios where delays are hard to predict and we propose a protocol for achieving oblivious transfer over the BDDC. We analyze the security of our construction in the semi-honest setting, showing that our realization of OT substantially decreases the protocol sensitivity to the user’s knowledge of the channel compared to solutions relying on other channel properties, and is very efficient for wide ranges of delay probabilities. The flexibility and generality of the model opens the way for future implementation in media where delays are a fundamental characteristic

Effect of reducing 3.2% dietary protein level on the growth performance and immunity of Nile tilapia (Oreochromis niloticus) with supplementation of multi amino acids

Reducing dietary protein content in fish feeds will reduce cost of production if growth performance can be maintained. In this study, we assessed the effects of reduced dietary protein content from 33.5% to 27.4% with ideal amino acids profile on the growth, immune parameters, intestinal microvilli length and total ammonia nitrogen discharge of tilapia. After 8 weeks of feeding, growth performance and feed efficiency were not affected by reducing dietary protein content from 33.5% to 30.3%, while fish fed 27.4% CP had the lowest weight gain. Total ammonia nitrogen discharged into the water 9 hours after the feeding was decreased by about 35%. Serum lysozyme activity, blood respiratory burst activity and serum ACH50 were not significantly affected by dietary protein content. Fold height, enterocyte height and microvillus height of proximal and middle intestine were significantly increased with reducing of dietary protein. Results indicated that 3.2% dietary protein content can be reduced, which had no effects on growth performance and immunity of Nile tilapia in practical diet

A Topological Study of Chaotic Iterations. Application to Hash Functions

International audienceChaotic iterations, a tool formerly used in distributed computing, has recently revealed various interesting properties of disorder leading to its use in the computer science security field. In this paper, a comprehensive study of its topological behavior is proposed. It is stated that, in addition to being chaotic as defined in the Devaney's formulation, this tool possesses the property of topological mixing. Additionally, its level of sensibility, expansivity, and topological entropy are evaluated. All of these properties lead to a complete unpredictable behavior for the chaotic iterations. As it only manipulates binary digits or integers, we show that it is possible to use it to produce truly chaotic computer programs. As an application example, a truly chaotic hash function is proposed in two versions. In the second version, an artificial neural network is used, which can be stated as chaotic according to Devaney

Relation of a New Interpretation of Stochastic Differential Equations to Ito Process

Stochastic differential equations (SDE) are widely used in modeling stochastic dynamics in literature. However, SDE alone is not enough to determine a unique process. A specified interpretation for stochastic integration is needed. Different interpretations specify different dynamics. Recently, a new interpretation of SDE is put forward by one of us. This interpretation has a built-in Boltzmann-Gibbs distribution and shows the existence of potential function for general processes, which reveals both local and global dynamics. Despite its powerful property, its relation with classical ones in arbitrary dimension remains obscure. In this paper, we will clarify such connection and derive the concise relation between the new interpretation and Ito process. We point out that the derived relation is experimentally testable.Comment: 16 pages, 2 figure

Direct Measurements of the Branching Fractions for D0→K−e+νeD^0 \to K^-e^+\nu_e and D0→π−e+νeD^0 \to \pi^-e^+\nu_e and Determinations of the Form Factors f+K(0)f_{+}^{K}(0) and f+π(0)f^{\pi}_{+}(0)

The absolute branching fractions for the decays $D^0 \to K^-e ^+\nu_e$ and $D^0 \to \pi^-e^+\nu_e$ are determined using $7584\pm 198 \pm 341$ singly tagged $\bar D^0$ sample from the data collected around 3.773 GeV with the BES-II detector at the BEPC. In the system recoiling against the singly tagged $\bar D^0$ meson, $104.0\pm 10.9$ events for $D^0 \to K^-e ^+\nu_e$ and $9.0 \pm 3.6$ events for $D^0 \to \pi^-e^+\nu_e$ decays are observed. Those yield the absolute branching fractions to be $BF(D^0 \to K^-e^+\nu_e)=(3.82 \pm 0.40\pm 0.27)$ and $BF(D^0 \to \pi^-e^+\nu_e)=(0.33 \pm 0.13\pm 0.03)$. The vector form factors are determined to be $|f^K_+(0)| = 0.78 \pm 0.04 \pm 0.03$ and $|f^{\pi}_+(0)| = 0.73 \pm 0.14 \pm 0.06$. The ratio of the two form factors is measured to be $|f^{\pi}_+(0)/f^K_+(0)|= 0.93 \pm 0.19 \pm 0.07$.Comment: 6 pages, 5 figure